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RNA to DNA Amplification
Directory > Science > Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR > RNA to DNA Amplification

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Improvements in quantitative real-time RT-PCR with QIAGEN Omniscript and Sensiscript RTs (PDF file)
Evalutaion of two sets of reagents for Real Time Reverse Transcriptase PCR to determine gene
expression. By S Ullmann and co-workers, at the manufacturer of the reagents.
http://www.qiagen.com/literature/qiagennews/0201/1016749_QNews22001p13-16.pdf
Reviews Rating: Not yet Rated Whois Check

RT-PCR Methodology
Animation to illustrate the way reverse transcriptase PCR (RT-PCR) produces DNA copies of an RNA
template, from Cold Spring Harbor Laboratory Learning Center, New York.
http://www.bio.davidson.edu/courses/Immunology/Flash/RT_PCR.html
Reviews Rating: Not yet Rated Whois Check

Reverse transcriptase-PCR
Protocol by Ignacio Rodriguez at the National Eye Institue, NIH, USA.
http://micro.nwfsc.noaa.gov/protocols/revtrpcr.html
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Effect of Primer Purity on the Banding Patterns of Differential Display Polymerase Chain Reaction
Article: L Wen: J Biomol Tech 2000;11:87-91.
http://www.abrf.org/JBT/2000/june00/jun00wen.html
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Differential Display PCR
Used to study differences in gene expression using RT PCR. From: U.S. National Coordination Program
for Swine Gene Mapping.
http://www.genome.iastate.edu/edu/newtech/ddpcr.html
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Differential Display of Cotton Transcripts
Detailed protocol, based on P Song, E Yamamoto, R Allen: Plant Mol. Biol. Rep. 13(1995):174-181.
From the web site of the GrainGenes ACEDB Database.
http://wheat.pw.usda.gov/~lazo/methods/lazo/ddrtpcr.html
Reviews Rating: Not yet Rated Whois Check

A Critical Evaluation of Differential Display as a Tool to Identify Genes Involved in Legume Nodulation
Article: S Lievens, S Goormachtig, M Holsters: Nuc Acids Res. 2001: 29(17) 3459-3468.
http://nar.oupjournals.org/cgi/content/full/29/17/3459
Reviews Rating: Not yet Rated Whois Check

Applications of Differential-Display Reverse Transcription-PCR to Molecular Pathogenesis and Medical Mycology
Survey article: J Sturtevant: Clin. Microbiol. Rev. 2000: 13(3) 408-427.
http://cmr.asm.org/cgi/content/full/13/3/408
Reviews Rating: Not yet Rated Whois Check

About mRNA Differential Display
Description of the technique and listing of materials supplied by GenHunter.
http://www.genhunter.com/products/differential/
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