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Search Results Long pcr Protocol
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
Protocol and guidelines for choice of conditions for pcr of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA
http://twod.med.harvard.edu/labgc/estep/longpcr_protocol.html
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Inverse pcr for PAC-end sequencing
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
To generate pcr fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA.
http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/prot2.html
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pcr Protocols
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
Protocols and technical hints, particularly for reverse transcription pcr, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University
http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html
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Degenerate pcr
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
The identification of novel members of gene families by pcr using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College.
http://www.dartmouth.edu/artsci/bio/ambros/protocols/other/koelle/degenerate_pcr.html
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Detection of Asymmetric pcr Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
In asymmetric pcr, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence.
http://imd-www.es.hokudai.ac.jp/~kinjo/fapcr/fapcrout.html
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pcr Troubleshooting
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
Limited to conventional straight forward pcr. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center.
http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html
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DNALC: pcr Animation
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
An animation explaining how the Polymerase Chain Reaction (pcr) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory.
http://www.dnalc.org/shockwave/pcranwhole.html
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pcr and multiplex pcr guide
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
Discussions of the parameters influencing the pcr reaction and some pcr and multiplex pcr applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center.
http://www.info.med.yale.edu/genetics/ward/tavi/Guide.html
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A Rapid DNA Minipreparation Method Suitable for AFLP and Other pcr Applications (PDF document)
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr
Preparation of DNA from plant tissues suitable for pcr methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis.
http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb17/17053-1.pdf
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Gene Quantification and real-time pcr or kinetic RT-pcr
Category: Science>Biology>Biochemistry and Molecular Biology>Methods and Techniques>pcr>Real Time pcr
News- and overview site on technical aspects of gene expression analysis with real-time RT-pcr and competitive RT-pcr from the Technical University of Munich, Germany
http://www.wzw.tum.de/gene-quantification/
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