Directory > Science > Biology > Biochemistry and Molecular Biology > Methods and Techniques > PCR Biosource molecular methods booklet 56 page booklet on oligonucleotides, PCR and RT-PCR, from Biosource (company). http://www.biosource.com/content/techCornerContent/methodPDFs/MolecularMethods.pdf Reviews Rating: Not yet Rated
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Innovative Tools for Amplification Notes about PCR applications, principles, research results, from Roche. http://www.roche-applied-science.com/sis/amplification/ Reviews Rating: Not yet Rated
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Optimizing PCR Protocols Brief guidelines. From the Jackson Laboratory, University of Maine, USA. http://www.jax.org/imr/optimize_pcr.html Reviews Rating: Not yet Rated
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Quantitative PCR Protocol From the Jackson Laboratory, University of Maine, USA. http://www.jax.org/cyto/quanpcr.html Reviews Rating: Not yet Rated
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Protocols Online: PCR Protocols Extensive collection of PCR protocols and methods from Protocol On Line. http://www.protocol-online.org/prot/Molecular_Biology/PCR/ Reviews Rating: Not yet Rated
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Attotron Biosensor Corporation R & D company for development of biosensors and related products for the research and educational markets. http://www.attotron.com Reviews Rating: Not yet Rated
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MJ Research Manufactures and sells products for molecular biology including thermal cyclers and sequencers / genotypers. http://www.mjr.com/ Reviews Rating: Not yet Rated
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PCR animated Animation illustrating the principle of PCR, from the University of Ghent, Belgium. http://allserv.rug.ac.be/~avierstr/principles/pcrani.html Reviews Rating: Not yet Rated
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PCR Animation Explaining how Polymerase Chain Reaction (PCR) works. From Dolan DNA Learning Center, Cold Spring Harbor Laboratory, USA. http://www.dnalc.org/Shockwave/pcranwhole.html Reviews Rating: Not yet Rated
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RAPD analysis with P. infestans Protocol for Randomly Amplified Polymorphic DNA, by H Judelson, University of California, Riverside, USA. http://www.fgsc.net/methods/Judel.html Reviews Rating: Not yet Rated
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RAPD's Brief description of Random Amplified Polymorphic DNA, from Oklahoma State University, USA. http://opbs.okstate.edu/~melcher/MG/MGW1/MG11127.html Reviews Rating: Not yet Rated
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RAPD PCR RAPD stands for Random Amplification of Polymorphic DNA, where the target sequence(s) (to be amplified) is unknown.Brief description, from Rutgers University, USA. http://avery.rutgers.edu/WSSP/StudentScholars/project/archives/onions/rapd.html Reviews Rating: Not yet Rated
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3' RACE PCR This technique is used to obtain the 3'end of a cDNA. Protocol by R Hastings, Biochemistry, University of Nottingham Medical School, UK. http://www.nottingham.ac.uk/~mbzspd/methods/3RACE_PCR.html Reviews Rating: Not yet Rated
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Detection of Single Nucleotide Mutations in Wheat Using Single Strand Conformation Polymorphism Gels (PDF file) P Martins-Lopez, H Zhang, R Koebner, Plant Mol. Biol. Reporter 19(2001): 159-162. From National Research Coouncil Canada. http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb19/R01-013.pdf Reviews Rating: Not yet Rated
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Mutation Detection Systems - SSCP Analysis Discussion of the technique and variations, literature references. By S McQuaid, Institute of Child Health, University College London, UK. http://www.ich.ucl.ac.uk/cmgs/sscp.htm Reviews Rating: Not yet Rated
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Cleaved Amplified Polymorphic Sequence Illustration of principle. CAPS corresponds to RFLP. From Corvallis Forestry Research Community, Corvallis, USA. http://www.fsl.orst.edu/tgerc/PCRCourse/caps.jpg Reviews Rating: Not yet Rated
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Detection of Point Mutations by RFLP of PCR Amplified DNA Sequences RFLP = Restriction Fragment Length Polymorphism. By Alexander Binder 1997. http://www-ang.kfunigraz.ac.at/~binder/thesis/node64.html Reviews Rating: Not yet Rated
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Nematode ITS1 Size Variation Examples of Restriction Fragment Length Polymorphism (RFLP)electrophoresis slabs for different nematodes. http://nematode.unl.edu/its_id/EXAMPLES/index.htm Reviews Rating: Not yet Rated
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RFLP Definition RFLP = Restriction Fragment Length Polymorphism, from FDA http://vm.cfsan.fda.gov/~frf/rflp.html Reviews Rating: Not yet Rated
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C. elegans Gene Knockout Project Protocols including standard nested PCR, from the Biotechnology Laboratory, University of British Columbia. http://ko.cigenomics.bc.ca/ Reviews Rating: Not yet Rated
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Improved diagnosis of porcine proliferative enteropathy caused by Lawsonia intracellularis using polymerase chain reaction-enzyme-linked oligosorbent assay P Zhang, CJ Gebhart, D Burden, GE Duhamel: Molecular and Cellular Probes, 14(2) 101-108, on the site of BT&C, Inc. http://www.btc-bti.com/pcrelisa.htm Reviews Rating: Not yet Rated
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PCR Amplification of cDNA Segments by 2 Stage Nested PCR Protocol from the method database of NIH, USA. http://www.ncbi.nlm.nih.gov/SNP/snp_viewTable.cgi?type=method&method_id=555 Reviews Rating: Not yet Rated
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The "Poison Primer" Technique for Enhancing Detection of Small Deletions in Mutant Libraries This method works because the poison allows the formation of deletion products but titers out full-sized products. From the Biotechnology Laboratory, University of British Columbia. http://ko.cigenomics.bc.ca/poison1.html Reviews Rating: Not yet Rated
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Multiplex PCR A new PCR strategy that enables the amplification of multiple DNA targets in one run. From Boston University. http://www.bu.edu/research/Features/scicoalition_01/twist.html Reviews Rating: Not yet Rated
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Effect of PCR Buffer on multiplex PCR (PDF) Multiplex PCR employs different primer pairs in the same amplification reaction. This requires extensive optimization of annealing conditions. From Quiagen (company) http://www.qiagen.com/literature/brochures/pcr/pdf/pcrcha03.pdf Reviews Rating: Not yet Rated
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Molecular Staging, Inc. Company promoting diagnostic tests for genetic analysis; rolling circle amplification technology (RCAT), developed for amplifying signals from DNA, RNA and proteins, from Westport, CT. http://www.molecularstaging.com/Pages/RCAToverview_.html Reviews Rating: Not yet Rated
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T-DNA Generated Enhancer Traps in Arabidopsis Application of inverse PCR, partial genomic libraries and TAIL-PCR in cloning flanking, at the Department of Biological Sciences, Dartmouth College, Hanover, NH. http://www.dartmouth.edu/~tjack/ Reviews Rating: Not yet Rated
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Inverse PCR For use with Snyder mTn-lacZ/LEU2 Based Mutagenesis Protocol by M McMurray, Fred Hutchinson Cancer Research Center, Seattle, Wa. USA. http://www.fhcrc.org/labs/gottschling/misc/ipcr.html Reviews Rating: Not yet Rated
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Inverse PCR for PAC-end sequencing To generate PCR fragments that contain the ends of PAC inserts that can be sequenced. Protocol by B Barbazuk, Washington University Zebrafish Genome Resources Project, USA. http://www.genetics.wustl.edu/fish_lab/frank/cgi-bin/fish/prot2.html Reviews Rating: Not yet Rated
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Inverse PCR and Cycle Sequencing of P Element Insertions for STS Generation Step by step protocol, by EJ Rehm, Berkeley Drosophila Genome Project, USA. http://www.fruitfly.org/about/methods/inverse.pcr.html Reviews Rating: Not yet Rated
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Hot Start PCR Description of the technique and the reasons to use it, from the web site of Bio Can, a company manufacturing materials for PCR. http://www.biocan.com/mbio/hot_tag.htm Reviews Rating: Not yet Rated
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What is "Hot-start" PCR? Principle and description of the technique, from the web site of the Weizmann Institute, Rehovot, Israel. http://bip.weizmann.ac.il/mb/bioguide/pcr/PCRHot-start.html Reviews Rating: Not yet Rated
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Degenerate PCR An introduction with principle, applications and description of the technique. From the web site of Kebangsaan University, Malaysia. http://cgat.ukm.my/protease/degpcr.html Reviews Rating: Not yet Rated
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GenHunter Manufacturer of material for differential display PCR in Nashville, Tenn USA. http://www.genhunter.com Reviews Rating: Not yet Rated
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Rational primer design greatly improves differential display-PCR (DD-PCR) Article: D Graf, AG Fisher, M Merkenschlager: Nucl. Acids Res. 25:11 2239-2240. http://nar.oupjournals.org/cgi/content/full/25/11/2239 Reviews Rating: Not yet Rated
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Random Arbitrarily Primed PCR Protocol for RAP-PCR to study prokaryotic gene expression, from the Enterococcus Research Site, University of Oklahoma, USA. http://www.enterococcus.ouhsc.edu/random_pcr.asp Reviews Rating: Not yet Rated
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Inverse PCR protocol step by step protocol, from the web site of the Department of Biology, University of Michigan, USA. http://www.biology.lsa.umich.edu/research/labs/maddock/protocols/PCR/inverse_pcr_protocol.html Reviews Rating: Not yet Rated
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PCR Protocol Detailed PCR protocol from the web site of the Department of Biology, University of Michigan, USA. http://www.biology.lsa.umich.edu/research/labs/maddock/protocols/PCR/general_pcr_protocol.html Reviews Rating: Not yet Rated
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Anchor Probes for Comparative Mapping of Grass Species Article in which probes from different libraries were used to hybridize seven cereals at the Department of Plant Breeding and Biometry, Cornell University, NY. http://greengenes.cit.cornell.edu/anchors/ Reviews Rating: Not yet Rated
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Single tube confirmation PCR protocol For characterization colonies of transformed clones of Saccharaomyces, from the web site of the Stanford Genome Technology Center, Palo Alto, CA, USA. http://www-sequence.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html Reviews Rating: Not yet Rated
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Standard PCR protocols From Molecular Biology Techniques Manual, from the web site of the University of Cape Town, South Africa. http://web.uct.ac.za/microbiology/pcrcond.htm Reviews Rating: Not yet Rated
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Alu-PCR Hybridization A report and protocol, from Fondation Jean Dausset. http://www.cephb.fr/manips_alupcr.html Reviews Rating: Not yet Rated
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Detection of Asymmetric PCR Products in Homogeneous Solution by Fluorescence Correlation Spectroscopy In asymmetric PCR, the low concentration primer is quantitatively incorporated into double stranded DNA after an appropriate number of cycles. If this primer is fluorescent labelled, the dsDNA can be quantitated from the fluorescence. http://imd-www.es.hokudai.ac.jp/~kinjo/fapcr/fapcrout.html Reviews Rating: Not yet Rated
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A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications (PDF document) Preparation of DNA from plant tissues suitable for PCR methods including AFLP, article by DH CHEN and PC RONALD Department of Plant Pathology, University of California, Davis. http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb17/17053-1.pdf Reviews Rating: Not yet Rated
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Which DNA Marker for Which Purpose? Compendia of the Research Project "Development, optimisation and validation of molecular tools for assessment of biodiversity in forest trees", European Union DGXII Biotechnology FW IV Research Programme. From the web site of the University Library, Göttingen. http://webdoc.sub.gwdg.de/ebook/y/1999/whichmarker Reviews Rating: Not yet Rated
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PCR Troubleshooting Limited to conventional straight forward PCR. Page designed and maintained by Octavian Henegariu on the web site of the Yale - New Haven Medical Center. http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html Reviews Rating: Not yet Rated
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Optimizing DNA Amplification Protocols using the Eppendorf Mastercycler Basic discussion of the optimization of PCR, from the web site of Brinkmann Instruments. http://www.brinkmann.com/PCR_appl_protocolsMC.asp Reviews Rating: Not yet Rated
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PCR primer design and reaction optimisation Article by Ed Rybicki, Department of Molecular and Cell Biology, University of Cape Town in: Molecular Biology Techniques Manual, on the web site of the University of Cape Town. http://web.uct.ac.za/microbiology/pcroptim.htm Reviews Rating: Not yet Rated
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PCR and multiplex PCR guide Discussions of the parameters influencing the PCR reaction and some PCR and multiplex PCR applications, by Octavian Henegariu on the web site of the Yale - New Haven Medical center. http://www.info.med.yale.edu/genetics/ward/tavi/Guide.html Reviews Rating: Not yet Rated
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Adjuvants in PCR Reactions Brief discussion of additives to improve amplification efficiency and specificity of PCR, by Octavian Henegariu, Yale-New Haven Medical Center. http://info.med.yale.edu/genetics/ward/tavi/p16.html Reviews Rating: Not yet Rated
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Optimizing Multiplex and LA-PCR with Betaine LA-PCR = "long and accurate PCR". Article by PN Hengen in TIBS June 1997. http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jun97.html Reviews Rating: Not yet Rated
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Thermostable DNA Polymerases Discussion of their origin and briefly their properties. From the web site of Colorado State University. http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/enzymes/hotpolys.html Reviews Rating: Not yet Rated
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Life at High Temperatures Article by TD Brock from the web site of Department of Bacteriology, University of Wisconsin - Madison on the thermophilic organisms in Yellowstone hot springs, including Thermus aquaticus from which Taq polymerase was obtained. http://www.bact.wisc.edu/Bact303/b27 Reviews Rating: Not yet Rated
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PCR protocols Brief protocols for PCR in use at the Genome Sequencing Center of the Washington University in St Louis Medical School. http://genome.wustl.edu/tools/protocols/index.php?fin4=1 Reviews Rating: Not yet Rated
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Fidelity of DNA Polymerases for PCR Article by PN Hengen from TIBS 1995 http://www.lecb.ncifcrf.gov/~pnh/papers/TIBS/aug95.html Reviews Rating: Not yet Rated
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Wayward PCR primers Article by PN Hengen from TIBS 1995 on the loss of activity of PCR primers with time. http://www-lecb.ncifcrf.gov/~pnh/papers/TIBS/jan95.html Reviews Rating: Not yet Rated
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Kary Mullis Inventor Profile of Kary Mullis, the originator of PCR, from the National Inventors Hall of Fame web site. http://www.invent.org/hall_of_fame/109.html Reviews Rating: Not yet Rated
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Polymerase Chain Reaction Popular survey article by Mark V. Bloom, DNA Learning Center, Cold Spring Harbor Laboratory, from the web site of the US National Health Museum. http://www.accessexcellence.com/RC/CT/polymerase_chain_reaction.html Reviews Rating: Not yet Rated
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What the Heck is PCR? Popular description of the PCR technique by John C Brown, University of Kansas 1995. http://people.ku.edu/~jbrown/pcr.html Reviews Rating: Not yet Rated
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Ten things that can kill your PCR About the proper choice of reaction conditions in PCR. Article by Peter Frame published on the Biowire web site. http://www.biowire.com/nucleus/nucleus_1_1.jsp Reviews Rating: Not yet Rated
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Technical manual of PCR Intended for specialists planning PCR procedures in their laboratories. From the University of Cape Town updated 2001. http://web.uct.ac.za/microbiology/pcr.htm Reviews Rating: Not yet Rated
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Tavi's PCR protocols A page describing the main parameters and trouble-shooting in PCR. The page is somewhat dated (updated 1997) but still useful. http://info.med.yale.edu/genetics/ward/tavi/PCR.html Reviews Rating: Not yet Rated
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Rep-PCR Genomic Fingerprinting Bacteria are characterized by Rep-PCR fingerprinting using primers corresponding to naturally occurring repetitive sequences in the interspersed regions. http://www.msu.edu/user/debruijn/ Reviews Rating: Not yet Rated
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Principle of PCR Applications in work on aging of Caenorhabditis elegans and phylogeny of nematodes, by Andy Vierstraete, Department of Biology, University of Ghent, Belgium. http://allserv.rug.ac.be/~avierstr/principles/pcr.html Reviews Rating: Not yet Rated
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Polymerase Chain Reaction (PCR) A graphic description of the principle of PCR from the US National Health Museum web site. http://www.accessexcellence.org/AB/GG/polymerase.html Reviews Rating: Not yet Rated
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The Polymerase Chain Reaction A popular description of the way it works, its importance in different connections, legal aspects. Authored by TM Powledge at the Federation of the American Societies of Experimental Biology. http://www.faseb.org/opar/bloodsupply/pcr.html Reviews Rating: Not yet Rated
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PCR Technology An introduction by Connie Veilleux from the US National Health Museum website. http://www.accessexcellence.org/LC/SS/PS/PCR/PCR_technology.html Reviews Rating: Not yet Rated
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PCR Protocols Protocols and technical hints, particularly for reverse transcription PCR, somewhat outdated, compiled by Dr Jack Vanden Heuvel, Department of Veterinary Science and Molecular Toxicology Program, Penn State University http://www.cas.psu.edu/docs/CASDEPT/VET/jackvh/jvhpcr.html Reviews Rating: Not yet Rated
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PCR Project Presentations from the University of California at Berkley on PCR, both current research reports and reviews. http://sunsite.berkeley.edu/biotech/pcr/ Reviews Rating: Not yet Rated
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PCR method protocols Protocols for PCR posted by the Helen Donis-Keller Laboratory. http://hdklab.wustl.edu/lab_manual/pcr/pcrcontents.html Reviews Rating: Not yet Rated
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Kary B. Mullis - Autobiography The originator of PCR, from the Nobel e-museum web site. http://www.nobel.se/chemistry/laureates/1993/mullis-autobio.html Reviews Rating: Not yet Rated
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HiFi DNA HiFi DNA is a company selling a DNA polymerase for PCR at low temperature giving accurate replication of certain sequences where Taq fails. http://www.hifidna.com/ Reviews Rating: Not yet Rated
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General Notes on Primer Design in PCR Article on the influence of the design of the primer on the outcome of PCR, by Dr Vincent R. Prezioso, published on the domain of Brinkmann Instruments. http://www.brinkmann.com/PCR_appl_primer.asp Reviews Rating: Not yet Rated
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DNALC: PCR Animation An animation explaining how the Polymerase Chain Reaction (PCR) works, from the Dolan DNA learning center, Cold Spring Harbor Laboratory. http://www.dnalc.org/shockwave/pcranwhole.html Reviews Rating: Not yet Rated
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Long PCR Protocol Protocol and guidelines for choice of conditions for PCR of long sequences (10 kb or larger). From Genetics Dept., Harvard Medical School, Boston, MA, USA http://twod.med.harvard.edu/labgc/estep/longPCR_protocol.html Reviews Rating: Not yet Rated
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Degenerate PCR The identification of novel members of gene families by PCR using degenerate primers is described and protocols given. Article by Michael Koelle 1996 on the web site of Dartmouth College. http://www.dartmouth.edu/artsci/bio/ambros/protocols/other/koelle/degenerate_PCR.html Reviews Rating: Not yet Rated
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BioGuide - PCR Basics of hot start, touchdown, AP and RAPD, primer selection, design programs and references, from the Weizman Institute of Science, Rehovot, Israel. http://bip.weizmann.ac.il/mb/bioguide/pcr/contents.html Reviews Rating: Not yet Rated
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The web guide of PCR List of links and forum on the subject and related methodology. Set up and maintained by SJ Krivokapich, National University of Misiones, Argentina. http://www.pcrlinks.com/ Reviews Rating: Not yet Rated
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Protocol for T-RFLP by Capillary Electrophoresis Detailed protocol for terminal restriction fragment length polymorphism (T-RFLP), which allows the fingerprinting of a community by analyzing the polymorphism of a certain gene. Article by V Grüntzig, B Stres, HL Ayala del Río and JM Tiedje, Center for Microbial Ecology, Michigan State University, USA http://rdp.cme.msu.edu/html/t-rflp_jul02.html Reviews Rating: Not yet Rated
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FISH Guide and Troubleshooting Links to pages describing influential parameters, with guides on PCR, RT-PCR and multiplex PCR reactions, Taq, FISH, CM-FISH, TM-FISH, microarrays, CCK, slide prep and labeling, maintained by Octavian Henegariu from Yale University, New Haven, CT. http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html Reviews Rating: Not yet Rated
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Design Oligonunucleotide Primers Interface for scanning DNA sequences for restriction sites, without changing encoded protein created. Includes FAQ, table of enzymes and links to tools, maintained at the University of Waterloo, ON, Canada. http://sciborg.uwaterloo.ca/~mpalmer/silent_mutations.html Reviews Rating: Not yet Rated
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Roe Laboratory Protocols Molecular biological protocols, mostly PCR related used by Bruce A. Roe at the Dept. of Chemistry and Biochemistry, OU, Norman, OK. http://www.genome.ou.edu/proto.html Reviews Rating: Not yet Rated
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