| Directory > Science > Biology > Biochemistry and Molecular Biology > Methods and Techniques Protocols
Microsatellites and others techniques from the Savana River Ecology Laboratory, University of
Georgia.
http://www.uga.edu/srel/DNA_Lab/protocols.htm
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Bioscientific Research
Personal page with hybridization, extraction and plant expression vectors and library suggestions.
http://www.biosc.org/interest.htm
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Hirsh Lab Gel Documentation System
Description of an agarose gel set-up. Includes instructions for camera operation with a script,
with computer side image analysis from the University of Virginia, Charlottesville.
http://www.virginia.edu/biology/Fac/hirsh_gel/gel/
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ECR Browser
Multiple alignment and dynamic evolutionary conservation profile of several vertebrate genomes
including human, mouse, rat, frog and three fishes, developed LBNL, Berkley, California.
http://ecrbrowser.dcode.org/
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Clontechniques
Quarterly newsletter, with search to access new product information and applications research
updates and earlier articles from of Clontech, now in the BD BioSciences Group, at Palo Alto, CA.
http://www.bdbiosciences.com/clontech/archive/index.shtml
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Tronolab Website
Collection of protocols, maps and sequences regarding manipulation of lentiviral vectors, siRNA and
transgenic animals. Applications in HIV, stem cell and gene therapy research at University of
Geneva.
http://www.tronolab.com/
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Recombinant DNA
Preparing probe, cloning into plasmids, complementarity and hybridization, gel electrophoresis,
transfer to solid support and blotting variants, blocking, target detection PCR problems from
chapter in MIT hypertext handbook.
http://web.mit.edu/esgbio/www/rdna/rdnadir.html
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Beginning Laboratory Manual
Teaching manual to support the applied biotechnology programs at the University of Maryland,
Baltimore.
http://www.research.umbc.edu/~jwolf/method1.html
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Surgery Research
Collection of DNA microarray technology protocols, primarily for resident students at Stanford.
http://surgeryresearch.com/
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Home Page of Andy Vierstraete
The use of DNA techniques in phylogenesis. Explanations, protocols and animations from Andy
Vierstraete at the University of Ghent, Belgium.
http://allserv.ugent.be/~avierstr/
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BioProtocol
Internet project initiated in early 1992 as BRNI, bio.com includes PCR, hybridization and
microarray protocols.
http://bioprotocol.bio.com/protocolstools/
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biowww.net: Molecular Biology
Comprehensive trouble shooting archive, with protocol and newsgroup links, including cell biology,
immunology and genetics.
http://www.biowww.net/index.php/article/archive/9/
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Molecular Biology Protocols
Protocol collection, with interactive forum for over ten topics, covering DNA, PCR, RNA and
proteins techniques and links to online journals and bioinformatics resources, from the Northwest
Fisheries Science Center Microbial Pathogenesis Lab, Seattle, WA.
http://micro.nwfsc.noaa.gov/protocols/
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SNP Scoring
Overview of genotyping, detection and quantifying single nucleotide polymorphism, including the
application of template directed dye terminator incorporation assay. Offers database and links to
other resources from PerkinElmer Life Sciences, Boston, MA.
http://lifesciences.perkinelmer.com/areas/snps/
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Biopolymer Calculator
Calculates molecular weight, composition and concentration of nucleic acids and proteins, based on
provided sequence, dilution and absorbance measurement. Also melting temperatures for nucleic
acids. Maintained at Schepartz Laboratory of Chemical Biology, Yale, New Haven, CT.
http://paris.chem.yale.edu/extinct.html
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Owczarzy, Richard
Online calculations of nucleic acid extinction coefficients, melting temperatures, and links to
biophysics databases. Includes thesis, publications and contacts for commercial scientist in
Coralville, Iowa.
http://www.owczarzy.net/
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Reto's Buffers and Media Book
Alphabetical list for buffer recipes, including constituents for cultures, from personal page in
Switzerland.
http://www.ikp.unibe.ch/~rportman/buffersmedia.html
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Biomedpage
Collection of laboratory protocols, applications and links to publications in biomedicine and
molecular biology from author in Germany.
http://www.biomedpage.de/indexe.html
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Laboratory Dialysis Answers
Discussion board for laboratory dialysis tips and techniques, moderated by Spectrum Laboratories,
Inc.
http://www.labdialysischat.com/
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Comparative Genomic Hybridization
CGH is a molecular cytogenetic method of screening a tumor for genetic changes. CGH protocols,
images, publications, events and links maintained by the University Hospital Charité,
Humboldt-University of Berlin, Germany.
http://amba.charite.de/cgh/
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Molbiol.net
Resource for molecular biologists on methods, with feedback for user-directed evolution and links
to commercial suppliers from Jonathan D Rees, Oxford, UK.
http://www.molbiol.net/
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Molecular Cloning Manual
Subscription only source of laboratory techniques in DNA and protein science, to complement the
hard copy, giving scientists the benefit of both media from CSHL, NY.
http://www.molecularcloning.com/
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Practical Molecular Biology
Methods, information, programs and BioMail for molecular biologists, as joint venture between
Germany and Russia.
http://molbiol.ru/eng/
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Molecular Probes, Inc.
Handbook of fluorescence applications, bibliography, data tables and calculators for molecular
biology, information on courses and workshops from supplier in Eugene, OR and Leiden, Netherlands.
http://www.probes.com/resources/
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Assay of Protein Concentration
Different methods of determining protein concentration, as a resource for researchers and students
in biochemistry and molecular biology laboratories from University of Nebraska, Lincoln, NE.
http://www-class.unl.edu/biochem/protein_assay/
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MolBio-Spot
Richard Man-kit Yu's site where you can acquire various molecular biology techniques.
http://www.geocities.com/richyuhk/
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Patrick Beisser's Web Site
This site provides links to various sites relevant for molecular biology and bioinformatics. It
also contains personal pages such as photographs and recreational pages.
http://mapage.noos.fr/beisser/index.html
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Molecular Biology Protocols
Resources such as polymerase chain reaction, dedicated to method development. Part of internet
directory maintained by Horizon Scientific Press, Wymondham, UK.
http://highveld.com/protocols.html
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SciWeb Protocols and Reagents
Directory of molecular biology protocol links.
http://sciweb.com/features/protocols.cfm
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Comet Assay Interest Group
Forum for exchange of views on the single cell gel electrophoresis system, capable of detecting
single- and double-strand breaks, oxidative DNA base damage, and DNA, protein and drug
crosslinking. Includes related links.
http://cometassay.com
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iProtocol for Bioscience
Key protocols used in biochemistry, bioengineering, cytobiology, genetics and molecular biology at
MIT, Cambridge, MA.
http://iprotocol.mit.edu
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Basic Principles of Gene Cloning, Sequencing, & Analysis
Description of methods behind gene cloning, genetic engineering, DNA recombination and isolation,
including discussion of restriction enzymes and ligase.
http://www.geocities.com:80/SiliconValley/5504/biochem.html
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BBHelp
Collection of molecular biology techniques derived from help files of Dr. Harold Zappe at the
Department of Medical Microbiology, University of Cape Town, South Africa.
http://www.uct.ac.za/depts/mmi/bbhelp/intro.html
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Comprehensive Protocol Collection
Comprehensive collection of molecular biology and Nematode-related protocols, including many
standard biological assays & stainings.
http://www.dartmouth.edu/artsci/bio/ambros/protocols.html
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